Create Injection Sequence
Create Injection Sequence from PlateObj (Single Plate)
Create Injection Sequence from MultiPlate (Multiple Plates)
Usage
build_injec_seq(
plate,
method,
rep_DB = 2,
rep_ISblank = 1,
rep_suitability = 1,
rep_blank = 2,
repeat_std = 1,
repeat_qc = 1,
repeat_analyte = 1,
repeat_dqc = 1,
n_explore = 0,
blank_after_top_conc = TRUE,
blank_at_end = TRUE,
blank_every_n = NULL,
injec_vol,
descr = "",
prefix = Sys.Date(),
suffix = "1",
tray = 1,
conc_df = NULL,
grouped = TRUE
)
# S4 method for class 'PlateObj'
build_injec_seq(
plate,
method,
rep_DB = 2,
rep_ISblank = 1,
rep_suitability = 1,
rep_blank = 2,
repeat_std = 1,
repeat_qc = 2,
repeat_analyte = 1,
repeat_dqc = 1,
n_explore = 0,
blank_after_top_conc = TRUE,
blank_at_end = TRUE,
blank_every_n = NULL,
injec_vol,
descr = "",
prefix = Sys.Date(),
suffix = "1",
tray = 1,
conc_df = NULL,
grouped = TRUE
)
# S4 method for class 'MultiPlate'
build_injec_seq(
plate,
method,
rep_DB = 2,
rep_ISblank = 1,
rep_suitability = 1,
rep_blank = 2,
repeat_std = 1,
repeat_qc = 1,
repeat_analyte = 1,
repeat_dqc = 1,
n_explore = 0,
blank_after_top_conc = TRUE,
blank_at_end = TRUE,
blank_every_n = NULL,
injec_vol,
descr = "",
prefix = Sys.Date(),
suffix = "1",
tray = 1,
conc_df = NULL,
grouped = TRUE
)Arguments
- plate
PlateObj object
- method
choose method from database
- rep_suitability
Number of re-injections for suitability vial.
- repeat_std
number of re-injections for calibration standards. Default is 1.
- repeat_qc
number of re-injections for QC wells. Default is 1
- repeat_analyte
number of re-injections for unknown samples. Default is 1
- n_explore
A number of exploratory samples to be injected at the top of the entire sequence. Default is 0
- blank_after_top_conc
If TRUE, adding blank after high concentrations of standards and QCS.
- blank_at_end
If True, adding blank at the end of queue.
- blank_every_n
If no QCs, frequency of injecting blanks between analytes.
- injec_vol
volume of injection in micro liters.
- descr
Run description.
- prefix
string at the beginning of the filename. Default is today's date.
- suffix
string to be added to the end of the filename. Default is "1".
- tray
Location in sample manager.
- conc_df
data.frame matching compound name to a scaling factor. Maximum 20 compounds allowed.